Synthesis, characterization, cytotoxicity and antimycobacterial screening of some p-substituted benzyl thiosemicarbazones

The aim of present study is to synthesize a series of different p-substituted benzyl thiosemicarbazone and evaluate their cytotoxicity as well as their antimycobacterial activity. The chemical structures have been assigned by micro analysis and spectral data (UV-Vis, IR and H NMR). All the compounds have been tested for their in vitro cytotoxic activity against leukaemia cancer cell line K562 and cell viability was evaluated by MTT assay. All the synthesized compounds indicate significant dosedependent cytotoxicity in very low micro-molar range (IC50 3.015.67 μM). The antimycobacterial drug susceptibility testing (DST) have been performed against M. smegmatis using microtiter plate by resazurin reduction assay (REMA) with glycerol and acetate as two different carbon sources. Some of the compounds show promising (>95%) result with acetate as carbon source and are being considered for further studies.